Assay & Detection

(HRP) Polymer anti-Rabbit

NEW
(HRP) Polymer anti-Rabbit

Article No

KDB-10046

Application

IHC

Species Reactivity

rabbit

Size

100 ml / 1000 Tests

Product insert

Suggested protocols

Specifications

Additional Information The new lots of HRP-polymers contain a different red color which is more orange in order to distinguish HRP polymers and AP polymers. Properties and quality of the products will remain the same.
Application IHC
Article No KDB-10046
BioSite Brand BioSite Histo
Conjugation HRP
Description (HRP) Polymer anti-Rabbit
Supplier BioSite Histo
Notes Enzym polymer single reagents with horse radish peroxidase. Ready-to-use.
Product Type Assay & Detection
Protocol Paraffin-embedded tissue sections are first deparaffinised and rehydrated. Endogenous peroxidase activity in the tissue may cause non-specific staining. This enzyme activity can be blocked by incubation with 3% H2O2-solution (&S220;Peroxide block&S221;). Background staining caused by unspecific binding of the primary antibody or the secondary antibody in the HRP polymer is minimized by incubation with a protein blocking solution (&S220;Blocking Solution&S221;). This step can be omitted if the primary antibodies are diluted in an appropriate buffer. The next step is incubation with the specific primary antibody. After washing, the HRP-polymer is applied and incubated. Any excess of unbound HRP-polymer is thoroughly washed away after incubation. The addition of the chromogenic substrate starts the enzymatic reaction of the horse radish peroxidase which leads to colour precipitation where the primary antibody is bound. The colour can be observed with a light microscope. The chromogen used determines the colour. The chromogen AEC leads to the formation of a red-brown product of reaction at the place of the target antigen. The chromogen DAB forms a dark brown precipitate.
References Elias JM &S220;Immunohistopathology &S211; A practical Approach to Diagnosis&S221; ASCP Press 2003 Nadji M and Morales AR. Ann N.Y. Acad Sci 420:134-9, 1983 Omata M et al. Am J Clin Pathol 73(5): 626-32, 1980
Size 100 ml / 1000 Tests
Species Reactivity rabbit
Storage The solution should be stored at 2-8°C without furt her dilution. Please store the reagent in a dark place and do not freeze it. Under these conditions the solution is stable up to the expiry date indicated on the label. They should not be used after the expiry date. A positive and a negative control have to be carried out in parallel to the test material.
Technical Specifications Double stain polymer detection kit with one step. Ready-to-use.
Product Page Updated 2019-08-15T09:29:01.207Z

Shipping info

The delivery time for this item is approximately 7-10 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.