Assay & Detection

(AP) Polymer Bulk Kit

NEW
(AP) Polymer Bulk Kit

Article No

KDB-10002

Application

ICC, IHC

Size

1 Kit (100 ml / 1000 Tests)

Product insert

Suggested protocols

Specifications

Application ICC, IHC
Article No KDB-10002
BioSite Brand BioSite Histo
Conjugation AP
Description (AP) Polymer Bulk Kit
Supplier BioSite Histo
Notes High sensitivy polymer detection kits for mouse and rabbit primary antibodies. Ready-to-use Blocking Solution, PostBlock, Enzym Polymer.
Product Type Assay & Detection
Protocol 1. Blocking Solution (protein block, Reagent 1) (This step is optional.) 5 min. 2. Washing with wash buffer 1 x 2 min. 3. Primary antibody (optimally diluted) or negative control reagent 30-60 min. 4. Washing with wash buffer 3 x 5 min. 5. PostBlock (Reagent 2, yellow) 20 min. 6. Washing with wash buffer 3 x 5 min. 7. AP-polymer (Reagent 3, red) 30 min. 8. Washing with wash buffer 3 x 2 min. 9. Fast Red, Permanent AP Red, NBT/BCIP or New Fuchsin 5-15 min. (Controlling the colour intensity via light microscope is recommended.) 10. Stopping the reaction with distilled H2O when the desired colour intensity is attained 11. Counterstaining and blueing 12. Mounting: aqueous with Fast Red, permanent with Permanent AP Red, NBT/BCIP or New Fuchsin
References Elias JM “Immunohistopathology – A practical Approach to Diagnosis” ASCP Press 2003 Nadji M and Morales AR Ann N.Y. Acad Sci 420:134-9, 1983
Size 1 Kit (100 ml / 1000 Tests)
Storage The solutions should be stored at 2-8°C without fur ther dilution. Please store the reagents in a dark place and do not freeze them. Under these conditions the solutions are stable up to the expiry date indicated on the label. They should not be used after the expiry date.
Technical Specifications High sensitivy polymer detection kits for mouse and rabbit primary antibodies
Product Page Updated 2019-08-15T09:29:01.207Z

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