Article No
KBB-ULF0A2-96
Human ADAM15 ELISA Kit
Human ADAM15 ELISA Kit (96 Tests). Quantitate Human ADAM15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Assay & Detection
Specifications
| MW | 92959 MW |
| Additional Information | Disintegrin and metalloproteinase domain-containing protein 15 is an enzyme that in humans is encoded by the ADAM15 gene. The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as an EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Application | ELISA |
| Article No | KBB-ULF0A2-96 |
| Biosite Brand | Biosite ELISA |
| Country Availability | all |
| Conjugation | Unconjugated |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Description | Human ADAM15 ELISA Kit (96 Tests). Quantitate Human ADAM15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 8751 |
| Gene Symbol | ADAM15 |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: D207-T696 |
| Localization | Endomembrane system, Single-pass type I membrane protein. Cell junction, adherens junction. Cell projection, cilium, flagellum. Cytoplasmic vesicle, secretory vesicle, acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The |
| Notes | Human ADAM15 ELISA Kit (96 Tests). Quantitate Human ADAM15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml. |
| Alias Names | ADAM15 | ADAM 15 | MDC15 | MDC 15 | MDC-15 | Metargidin | Q13444 |
| Previous Article No | KBB-2521CB |
| Product Type | Assay & Detection |
| Protocol | Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human ADAM15 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, serum or plasma(heparin, EDTA) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each human ADAM15 standard solution and each sample be measured in duplicate. |
| Range | 62.5 pg/ml - 4,000 pg/ml |
| Sample Type | Cell Culture, Plasma, Serum |
| Sensitivity | <12 pg/ml |
| Shipping Information | BLUE ICE |
| Size | 96wells/kit |
| Species Reactivity | human |
| Storage | -20°C, RT |
| Technical Specifications | For quantitative detection of human ADAM15 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| UniProt Number | Q13444 |
| Product Page Updated | 2024-04-17T08:14:24.385Z |
