Article No
K033-H1
Catalase Colorimetric Activity Kit
Catalase Colorimetric Activity Kit
Assay & Detection
Specifications
| Application | Detection |
| Article No | K033-H1 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Components | Activity |
| Description | Catalase Colorimetric Activity Kit |
| Detection Method | Colorimetric |
| Supplier | Arbor Assays |
| Keywords | Oxidative Stress & Stress |
| Notes | Catalase Colorimetric Activity assay Kit |
| Product Type | Assay & Detection |
| Protocol | The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. Please read the complete kit insert before performing this assay. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Colorimetric Detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a pink-colored product. The colored product is read at 560 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in pink product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL. Five random adult human serum samples and five adult human plasma samples were diluted in Assay Buffer between 1:10 and 1:80 and run in the assay. |
| Range | The serum samples ranged from 6.04 to 128.3 U/mL with an average of 36.9 U/mL after adjusting for dilution. The plasma samples ranged from 11.4 to 157.1 U/mL with an average of 70.2 U/mL after adjusting for dilution. |
| Sensitivity | 0.052 U/mL |
| Shipping Information | BLUE ICE |
| Size | 2x96 well plate |
| Species Reactivity | cat/feline, cow/bovine, dog/canine, horse/equine, human, mammals, mouse, multi species, pig/porcine, rabbit, rat, sheep/ovine |
| Storage | 4°C |
| Technical Specifications | Catalase |
| Product Page Updated | 2024-03-04T10:32:24.675Z |
