Article No
182-HM1039-500UG
Neutrophils, Mouse, mAb NIMP-R14 – 500 µg
Neutrophils, Mouse, mAb NIMP-R14 – 500 µg
Antibodies Primary
Specifications
| Application | FC, FS, IA |
| Article No | 182-HM1039-500UG |
| Country Availability | SE, FI, DK, NO, EE, LV, LT |
| Clone | NIMP-R14 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | Neutrophils, Mouse, mAb NIMP-R14 – 500 µg |
| Supplier | Hycult biotechnology |
| Format | 0.5 mg 0.2 μm filtered protein G purified antibody solution in PBS with a concentration of at least 0.5 mg/ml. The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay. |
| Immunogen | Purified BALB/c mouse neutrophils |
| Isotype | IgG2b |
| Notes | The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. |
| Alias Names | Lymphocyte antigen 6 complex locus protein G6c, myeloid differentiation antigen Gr1 Gene name: Ly6g |
| Previous Article No | HM1039-FS, 182-HM1039-500UG, HM1039-500UG |
| Product Type | Antibodies Primary |
| Research area | Immunology |
| Shipping Information | Room Temperature |
| Size | 500 µg |
| Source / Host | rat |
| Species Reactivity | mouse |
| Storage | Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. |
| Substrate / Buffer | Quantities >500 µg will be send without preservative and carrier free. Quantities |
| Product Page Updated | 2024-04-05T09:42:44.568Z |
