<b>Recommended Starting Dilutions:</b><br>FACS: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line<br>IP: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line<br>WB: Positive Control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE Sample buffer. Do not heat/boil. Use under non-reducing conditions<br>Optimal dilutions should be determined experimentally by the researcher.
Source / Host
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4-8ºC. DO NOT FREEZE. Protect from light.
Substrate / Buffer
The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
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