Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining. In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. Immuno Wash Buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining.
No pH adjustment or additives are required when using this formulation. Simply dilute the concentrated 10-fold buffer with distilled or deionized water. A total of 10 liters of working rinsing solution can be prepared with 1 liter of the concentrated reagent. This buffer is also the reagent of choice for rinsing tissue sections that are subjected to immunocytochemical staining using conventional horizontal processing techniques.
Store at room temperature.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
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