Article No
GTX60456
Specifications
| Application | ELISA, FC, ICC, IF, IHC, WB |
| Article No | GTX60456 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | ASK1 antibody [2E4] |
| Supplier | GeneTex |
| Entrez Gene ID | 4217 |
| Gene Symbol | MAP3K5 |
| Immunogen | Purified recombinant fragment of human MAP3K5 expressed in E. Coli. |
| Isotype | IgG1 |
| Notes | Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008] |
| Product Type | Antibodies Primary |
| Protocol | WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
| Purification | Unpurified |
| Size | 100µl |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C, -80°C |
| UniProt Number | Q99683 |
| Product Page Updated | 2024-01-03T10:31:47.950Z |
