A-20 (mouse B lymphocyte; reticulum cell sarcoma) cell line slides
A-20 (B lymphocyte; reticulum cell sarcoma)Mouse A-20 cells were cultured in RPMI 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. The A-20 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A-20 cells. A follow-up heat dehydration process was done to ensure the attachment of A-20 cells and the stability of cellular proteins.