A549 (lung; carcinoma)Human A549 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The A549 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A549 cells. A follow-up heat dehydration process was done to ensure the attachment of A549 cells and the stability of cellular proteins.
Tissues & Slides
A549 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
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