Antigen Retrieval Solution

Antigen retrieval solution-enzymatic (trypsin)

Sample Preparation

Article No

GTX20970

Application

IHC

Size

1kit

Article No

GTX20970

Application

IHC

Size

1kit

Specifications

Application IHC
Article No GTX20970
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Control Tissue Infected lung
Description Antigen retrieval solution-enzymatic (trypsin)
Supplier GeneTex
Notes Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product used to be called ""Carezyme Trypsin Enzymatic Antigen Retrieval Solution"".
Product Type Sample Preparation
Protocol Deparaffinize tissues and hydrate to water. If necessary perform hydrogen peroxide block, was in water and rinse in PBS or TBS. The tissue section is now ready for trypsin digestion. Combine 1 part trypsin with 1 part buffer. Incubate for 5-10 miinutes at room temperature. Rinse well in PBS or TBS buffer and proceed with immunostaining procedure.This product utilises - color-coded buffer (rose) for easy identification. The buffer incorporates a color-coded pH indicator. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion. This product is provided as a two component, ready-to-use solution, and can be used at different concentrations: (1) 25 ml Concentrated liquid trypsin (2) 25 ml of trypsin buffer
Size 1kit
Storage 4°C
Product Page Updated 2024-01-03T10:31:47.950Z

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