AEC Chromogenic kit

Article No GTX16204
Product Type Sample Preparation
Application IHC
Format Liquid
Size 1 kit

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Specifications

Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Data Sheet Link http://www.genetex.com/Web/Product/ProductToPDF.aspx?No=GTX16204&Country=46
Description AEC Chromogenic kit
Supplier GeneTex Inc.
Notes AEC is one of the most important chromogenic reagents for peroxidase. The coloration which is showed to be fresh red, and applies to immunohistochemistry and various dot detection. Easy to solution in organic impregnant is its defect, to seal slide with water soluble sealing reagent in case of using AEC is recommend.
Protocol 1. After the peroxidase detection, wash 4 times with TBS or PBS and each time for 5 minutes.<br>2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.<br>3. Wash fully with distilled water to stop reaction. Counterstain with methyl blue for 3-5 minutes or nonalcoholic hematoxylin (Mayer's) if necessary.<br>4. Seal sllide with water soluble sealing reagent (Boster can provide), or glycerol. Observe with microscope. Counterstain with alcoholic hematoxylin is not recommended.
Size 1
Size Unit kit
Species Reactivity mouse
Storage Store at -20°C for one year.
Technical Specifications For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Product Page Updated 2019-01-17T12:56:28.093Z

Shipping info

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Species reactivity:

mouse mouse

Product insert:

Product data sheet

Datasheet

Suggested protocols:

IHC-P

IHC-P