Article No
GTX12139
Specifications
| Application | FC, ICC, IF, IHC, IP, MS, WB |
| Article No | GTX12139 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | 10AG2 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | IKK beta antibody [10AG2] |
| Supplier | GeneTex |
| Entrez Gene ID | 3551 |
| Gene Symbol | IKBKB |
| Immunogen | A full-length human IKKb recombinant protein was used to immunize mice (NP_001547). |
| Isotype | IgG1 |
| Notes | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Product Type | Antibodies Primary |
| Protocol | WB: 2 - 4 µg/ml. IHC-P: 1:10 - 1:500. FACS: 0.25 - 0.5 µg / 10⁶ cells. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications. |
| Purification | Protein G Purified |
| Size | 50µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | 4°C, -80°C |
| UniProt Number | O14920 |
| Product Page Updated | 2024-01-03T10:31:47.950Z |
