Chemicals & Biochemicals

(+)-Biotin N-hydroxysuccinimide ester

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(+)-Biotin N-hydroxysuccinimide ester

Article No

CDX-B0145-M250

Size

250 mg

Product insert

Specifications

CAS No 35013-72-0
MW 341.38
Article No CDX-B0145-M250
Description (+)-Biotin N-hydroxysuccinimide ester
Supplier Adipogen Life Sciences
Notes Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
Molecular Formula C14H19N3O5S
Alias Names (+)-Biotin N-succinimidyl ester; d-Biotin NHS ester; BNHS; Biotinyl-N-hydroxy-succinimide; N-Hydroxysuccinimidobiotin; N-Succinimidyl D-biotinate; NHS-Biotin; Biotin-Osu; NSC 345668
Product Type Chemicals & Biochemicals
Purity >98% (HPLC)
References (1) D.M. Boorsma, et al.; J. Microsc. 143, 197 (1986) | (2) P. Kongtawelert & P. Ghosh; Clin. Chem. Acta 195, 17 (1990) | (3) G. Paganelli, et al.; Int. J. Cancer 45, 1184 (1990) | (4) C. Wagener, et al.; Meth. Enzymol. 184, 518 (1990) | (5) B. Baumeister, et al.; Int. J. Pep. Res. Therap. 11, 139 (2005) | (6) G.T. Hermanson; Bioconjugate Techn. 2nd p506-545 (2008)
Size 250 mg
Stability Stable for at least 2 years after receipt when stored at -20°C.
Storage -20°C
Technical Specifications Chemical. CAS: 35013-72-0. Formula: C14H19N3O5S. MW: 341.38. Biotin succinimidyl ester is the most popular amine-reactive biotinylating agent for modifying proteins, peptides, antibodies, oligonucleotides, microbeads and other biological molecules. It forms irreversible amide bonds. Typically coupled to primary amine in the pH range 6.5-8.5. This cell-permable activated biotin derivative can be readily coupled to primary or secondary amines of analyte compounds or proteins under mild conditions. The resulting biotinylated derivatives have been widely used in streptavidin or avidin systems and labeling methods, with many applications in immunology and histochemistry. It is used to selectively label Escherichia coli cell envelope proteins in vivo. It preferentially labels outer membrane, periplasmic, and inner membrane proteins as well as a specific inner membrane marker protein (Tet-LacZ).
Product Page Updated 2019-07-12T09:17:06.689Z

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