Biotin-(5-fluorescein)-conjugate

Biotin-(5-fluorescein)-conjugate

Secondary Reagents

Article No

CDX-B0078-M005

Size

5 mg

Shipping Information

RT

Article No

CDX-B0078-M005

Size

5 mg

Shipping Information

RT

Specifications

CAS No 957494-27-8
MW 644.7
Article No CDX-B0078-M005
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Description Biotin-(5-fluorescein)-conjugate
Supplier Adipogen Life Sciences
Format Powder
Notes Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.|Chemical. CAS: 957494-27-8. Formula: C33H32N4O8S. MW: 644.7. Synthetic. Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
Molecular Formula C33H32N4O8S
Alias Names Biotin-4-Fluorescein; B4F; N-(Biotinylamidoethyl)-fluorescein-5-carboxamide; N-(Fluorescein-5-carbamidoethyl)biotinamide
Product Type Secondary Reagents
Purity >90%
Research Area Immunology
Shipping Information RT
Size 5 mg
Solubility Soluble in DMSO.
Stability Stable for at least 2 years after receipt when stored at -20°C.
Storage -20°C
Technical Specifications Chemical. CAS: 957494-27-8. Formula: C33H32N4O8S. MW: 644.7. Synthetic. Biotin-fluorescein conjugate for the fluorometric accurate measurement and quantification of avidin and streptavidin. Shows exceptionally fast and tight binding to avidin and streptavidin accompanied by strong quenching. Used in spectrophotometric streptavidin-biotin binding assays. May be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. Spectral Data: lambdaex 490nm; lambdaem 524nm in 0.1M Tris pH8.0.
Product Page Updated 2023-03-13T16:14:25.982Z

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