Sample Preparation

Direct-zol™ RNA MiniPrep Plus w/ Zymo-Spin™ IIICG Columns

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Direct-zol™ RNA MiniPrep Plus (50 Preps) w/ Zymo-Spin™ IIICG Columns (Capped)

Article No

BioSite-R2070

Application

RNA Purification

Size

50 Preps

Specifications

Application RNA Purification
Article No BioSite-R2070
Description Direct-zol™ RNA MiniPrep Plus (50 Preps) w/ Zymo-Spin™ IIICG Columns (Capped)
Supplier Zymo Research Europe GmbH
Notes The Direct-zol RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA (see below). The procedure is easy; Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA; No phase separation, precipitation, or post-purification steps are necessary; The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.
Product Type Sample Preparation
Research Area Molecular Biology
Size 50 Preps
Storage RT
Technical Specifications Compatibility: TRIzol, RNAzol, QIAzol, TriPure, TriSure and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent. Equipment needed: Microcentrifuge RNA size limits: RNAs ≥17 nucleotides. RNA storage: RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage. Sample sources: Any sample stored and preserved in TRI Reagent, TRIzol or similar. (animal cells, tissue, bacteria, yeast, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Product Page Updated 2019-06-04T15:07:06.527Z

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