The Direct-zol RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA (see below). The procedure is easy; Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA; No phase separation, precipitation, or post-purification steps are necessary; The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.
Compatibility: TRIzol, RNAzol, QIAzol, TriPure, TriSure and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent. Equipment needed: Microcentrifuge RNA size limits: RNAs ≥17 nucleotides. RNA storage: RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage. Sample sources: Any sample stored and preserved in TRI Reagent, TRIzol or similar. (animal cells, tissue, bacteria, yeast, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
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The delivery time for this item is approximately 4-7 business days, depending on the availability at manufacturer. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.