Antibiotics & Culture Media

100X 5-Fluoroorotic Acid (Liquid)

100X 5-Fluoroorotic Acid (Liquid)

Article No



DNA Purification


10 ml

Shipping Information




Application DNA Purification
Article No BioSite-F9003
Country Availability SE, FI, DK, NO, FO, GL
Description 100X 5-Fluoroorotic Acid (Liquid)
Supplier Zymo Research
Notes Convenient yeast genetic counter-selection agent.|Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection. The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and synthetic 5-FOA monohydrate powder. Both have been tested and validated on the basis of counter selection activity.
Product Type Antibiotics & Culture Media
Research Area Molecular Biology
Shipping Information BLUE ICE
Size 10 ml
Storage -20C
Product Page Updated 2022-05-06T12:11:26.294Z

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The delivery time for this item is approximately 4-7 business days, depending on the availability at manufacturer. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.