ZymoBIOMICS DNA Mini Kit (50 preps) Includes: [D4304] ZymoBIOMICS Miniprep Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1, [D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1
Zymo Research Europe GmbH
The ZymoBIOMICS™ DNA Mini Kit is designed for purifying DNA from a wide array of sample inputs (e.g. feces, soil, water, and biofilms), that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ZymoBIOMICS™ ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard. In addition, the ZymoBIOMICS™ DNA Mini kit is equipped with Zymo’s Proprietary OneStep™ PCR Inhibitor removal technology enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ purification technology results in superior yields of ultra-pure DNA ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.
Up to 25 µg total DNA can be eluted into 100 µl (50 µl minimum) ZymoBIOMICS™ DNase/RNase Free Water. Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, and 50 – 100 mg (wet weight) of fungal bacterial cells, biofilms and water. ZymoBIOMICS™ innovative lysis system enables complete homogenization/disruption of the microbial cells walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis it is recommend that the ZymoBIOMICS™ Microbial Community Standard is used for calibration of each bead beating device. Generally, post bead beating, genomic DNA has an average size of 15-20 kb depending on the initial quality of the sample making it amenable to Next-Gen sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield™
Product Page Updated
The delivery time for this item is approximately 4-7 business days, depending on the availability at manufacturer. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.