Article No
BPS-79982-1
Spike(SARS-CoV-2) Pseudotyped Lentivirus (Luc-eGFP Dual Reporter)
Spike(SARS-CoV-2) Pseudotyped Lentivirus (Luc-eGFP Dual Reporter)
Accessories
Specifications
| Additional Information | The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection. The SARS-CoV-2 Spike Pseudotyped Lentivirus (Luc-eGFP dual reporter) were produced by replacing the VSV-G fusion glycoprotein with SARS-CoV-2 Spike protein (Genbank Accession #QHD43416.1) as a surrogate viral envelope protein. These pseudovirions also contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. The luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells. Therefore, the Spike-mediated entry into the target cell can be conveniently measured via luciferase reporter activity or eGFP expression. The SARS-CoV-2 Spike pseudotyped lentivirus can be used in a cellular assay to measure the activity of neutralizing antibody against SARS-CoV-2. |
| Article No | BPS-79982-1 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | Spike(SARS-CoV-2) Pseudotyped Lentivirus (Luc-eGFP Dual Reporter) |
| Supplier | BPS Bioscience |
| Format | The functional titer of SARS-CoV-2 Spike Pseudotyped Lentivirus (~1-2 x 10^5 TU/ml) is significantly lower than VSV-G pseudotyped lentivirus (>10^7 TU/ml), although the amount of lenti particles are similar (>10<sup>9</sup> LP/ml). The exact titer is provided with each shipment. |
| Notes | The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection. The SARS-CoV-2 Spike Pseudotyped Lentivirus (Luc-eGFP dual reporter) were produced by replacing the VSV-G fusion glycoprotein with SARS-CoV-2 Spike protein (Genbank Accession #QHD43416.1) as a surrogate viral envelope protein. These pseudovirions also contain a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) driven by a CMV promoter. The luciferase and eGFP are coexpressed under the CMV promoter in the transduced cells. Therefore, the Spike-mediated entry into the target cell can be conveniently measured via luciferase reporter activity or eGFP expression. The SARS-CoV-2 Spike pseudotyped lentivirus can be used in a cellular assay to measure the activity of neutralizing antibody against SARS-CoV-2. |
| Alias Names | covid, covid-19, covid19, coronavirus, Severe acute respiratory Coronavirus Spike 2, pseudovirus, pseudovirion, lentivirus, dual reporter, neutralizing, ACE2, SARS-CoV-2 Spike |
| Product Type | Accessories |
| Shipping Information | -80°C (dry ice) |
| Size | 100 µl |
| Species Reactivity | virus |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
| Substrate / Buffer | The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS. |
| Product Page Updated | 2024-01-09T12:32:15.877Z |
