Article No
BPS-79414
CERK, FLAG-Tag (Rat) Recombinant
CERK, FLAG-Tag (Rat) Recombinant
Proteins & Peptides
Specifications
| MW | 61 kDa |
| Additional Information | Rat CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system. |
| Article No | BPS-79414 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | CERK, FLAG-Tag (Rat) Recombinant |
| Supplier | BPS Bioscience |
| Format | Aqueous buffer solution |
| Notes | Rat CERK, also known as ceramide kinase, GenBank Accession No. NM_00113486, 1-531 (end) with C-terminal Flag tag, MW= 61 kDa, expressed in baculovirus expression system. |
| Alias Names | ceramide kinase |
| Product Type | Proteins & Peptides |
| Protocol | CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI). |
| Shipping Information | -80°C (dry ice) |
| Size | 10 µg |
| Species Reactivity | rat |
| Storage | At least 6 months at –80°C. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol and 100ug/ml flag peptide |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
