14-3-3 alpha/beta Protein, His-tag (Mouse)

Article No BPS-79024
Product Type Proteins & Peptides
Application WB
Size 20 µg

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Specifications

MW 32 kDa
Additional Information Mouse 14-3-3 alpha/beta Protein, GenBank Accession No. NM_018753, full length, with N-terminal His tag, expressed in Sf9 insect cells using baculovirus. MW = 32 kDa.
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Description Mouse 14-3-3 alpha/beta Protein, GenBank Accession No. NM_018753, full length, with N-terminal His tag, expressed in Sf9 insect cells using baculovirus. MW = 32 kDa.
Supplier BPS Bioscience Inc
Notes 14-3-3 alpha/beta is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3 alpha/beta protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-alpha/beta interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-alpha/beta to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth.
Alias Names 14-3-3 alpha/beta, Ywhab; 1300003C17Rik; 14-3-3b
References 1. Yaffe, M B. et al: The structural basis for 14-3-3: phosphopeptide binding specificity. Cell 91: 961-971, 1997. _x000D_ _x000D_ _x000D_ _x000D_ 2. Shumway, S D. et al: 14-3-3-beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin. J. Biol. Chem. 278: 2089-2092, 2003.
Source / Host insect
Species Reactivity mouse
Storage Store product at -80°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles._x000D_
Substrate / Buffer 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Technical Specifications Western blot
UniProt Number Q9CQV8
Product Page Updated 2019-01-04T10:01:58.434Z

Shipping info

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Source/Host:

insect

Species reactivity:

mouse mouse

Suggested protocols:

Western Blot

Western Blot