Article No
BPS-78119
CD5 (Human) CRISPR/Cas9 Lentivirus (Integrating)
CD5 (Human) CRISPR/Cas9 Lentivirus (Integrating)
Accessories
Specifications
| Additional Information | The CD5 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human CD5 driven by a U6 promoter. The integrating lentivirus integrates randomly into the cellular genome to express both Cas9 and the sgRNA. Puromycin selection forces high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies may vary, depending on the cell type and the gene of interest. |
| Article No | BPS-78119 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | CD5 (Human) CRISPR/Cas9 Lentivirus (Integrating) |
| Supplier | BPS Bioscience |
| Notes | The CD5 CRISPR Lentiviruses are replication incompetent, HIV-based VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a CRISPR/Cas9 gene driven by an EF1a promoter, along with 5 sgRNA (single guide RNA) targeting human CD5 driven by a U6 promoter. The integrating lentivirus integrates randomly into the cellular genome to express both Cas9 and the sgRNA. Puromycin selection forces high expression levels of both Cas9 and the sgRNA, and can be used with the integrating lentivirus to quickly and easily achieve high knockdown efficiencies in a cell pool. Efficiencies may vary, depending on the cell type and the gene of interest. |
| Product Type | Accessories |
| Shipping Information | -80°C (dry ice) |
| Size | 500 µl x 2 |
| Species Reactivity | virus |
| Storage | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle. |
| Substrate / Buffer | The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS. |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
