Article No
BPS-78070
Additional Information | Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Jurkat cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. |
Article No | BPS-78070 |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
Description | Cas9 Expressing Jurkat Cell Pool |
Supplier | BPS Bioscience |
Notes | Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Jurkat cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. |
Alias Names | SpCas9 cell pool, CRISPR cell pool, Cas9 cell pool, Jurkat cells |
Product Type | Cells |
Shipping Information | -80°C (dry ice) |
Size | 2 vials |
Source / Host | human |
Species Reactivity | bacteria |
Storage | Immediately upon receipt, store in liquid nitrogen. |
Product Page Updated | 2024-01-09T13:23:45.900Z |