Article No
BPS-71099-2
IDH1 (R132H), FLAG-tag Recombinant
IDH1 (R132H), FLAG-tag Recombinant
Proteins & Peptides
Specifications
| MW | 48 kDa |
| Accession Number | NM_005896 |
| Additional Information | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). IDH1 homodimer is an NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, as well as in peroxisomal reactions that consume 2-oxoglutarate. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. |
| Article No | BPS-71099-2 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | IDH1 (R132H), FLAG-tag Recombinant |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 3417 |
| Format | Aqueous buffer solution |
| Gene Symbol | IDH1 |
| Notes | Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length [a.a. 1-414(end)] with Arg to His mutation on a.a. 132 and C-terminal FLAG-tag, MW= 48 kDa, expressed in Sf9 cells via a baculovirus expression system. |
| Alias Names | Isocitrate Dehydrogenase 1, IDH1, oxalosuccinate decarboxylase, IDP |
| Product Type | Proteins & Peptides |
| Protocol | IDH reductive activity was measured in 200 µl reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl<sub>2</sub>, 0.03% BSA, 1 mM alpha-Ketoglutarate, 10 ?M NADPH and IDH. Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200 M<sup>-1</sup>cm<sup>-1</sup>. |
| Research area | Metabolism |
| Shipping Information | -80°C (dry ice) |
| Size | 100 µg |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 0.04% Tween-20 and 20% glycerol. |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| UniProt Number | O75874 |
| Product Page Updated | 2024-01-09T12:32:15.877Z |
