Article No
BPS-71048
Sortase A, Heptamutant, His-Tag Recombinant
Sortase A, Heptamutant, His-Tag Recombinant
Proteins & Peptides
Specifications
| MW | 22 kDa |
| Accession Number | AF162687 |
| Additional Information | Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular "stapler" to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. This reaction is applicable to proteins in solution and on the living cell surface. |
| Article No | BPS-71048 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | Sortase A, Heptamutant, His-Tag Recombinant |
| Supplier | BPS Bioscience |
| Format | Aqueous buffer solution |
| Notes | Staphylococcus aureus sortase A Heptamutant (P94R, D160N, D165A, Y187L, E189R, K190E, K196T), a.a 26-206(end) GenBank Accession No. AF162687 with N-terminal His tag, MW = 22 kDa, expressed in an E. coli expression system. |
| Alias Names | SrtA, sortase A, heptamutant |
| Product Type | Proteins & Peptides |
| Shipping Information | -80°C (dry ice) |
| Size | 50 µg |
| Species Reactivity | bacteria |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 400 mM Imidazole and 20% glycerol |
| Technical Specifications | Suitable for use with in vitro protein ligation reactions. |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
