Article No
BPS-40065
BRAF, GST-tag Recombinant
BRAF, GST-tag Recombinant
Proteins & Peptides
Specifications
| MW | 66 kDa |
| Accession Number | NM_004333 |
| Additional Information | Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Article No | BPS-40065 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | BRAF, GST-tag Recombinant |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 673 |
| Format | Aqueous buffer solution |
| Gene Symbol | BRAF |
| Notes | Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system. |
| Alias Names | B-RAF1, BRAF, RAFB1 |
| Product Type | Proteins & Peptides |
| Protocol | Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl<sub>2</sub>, 0.1 mg/ml BSA)containing 2 µg inactive MEK1 (BPS #40075), 5 µM ATP, and 0.1 - 1.6 ng/µl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents. |
| Shipping Information | -80°C (dry ice) |
| Size | 10 µg |
| Source / Host | insect |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| UniProt Number | P15056 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
