Article No
BPS-40025
AMPK (A1/B1/G1), His-tags Recombinant
AMPK (A1/B1/G1), His-tags Recombinant
Proteins & Peptides
Specifications
| MW | ~146 kDa (complex) |
| Accession Number | NM_002733, NM_006251, NM_006253 |
| Additional Information | AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2). |
| Article No | BPS-40025 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Description | AMPK (A1/B1/G1), His-tags Recombinant |
| Supplier | BPS Bioscience |
| Format | Aqueous buffer solution |
| Notes | Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. The gene accession number for the three subunits (A1/B1/G1) is NM_006251, NM_006253, and NM_002733, respectively. This protein has been activated with CAMKK. |
| Alias Names | Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G1: PRKAG1, AMPKG, MGC8666 |
| Product Type | Proteins & Peptides |
| Protocol | Assay was done in Kinase_x000D_ buffer containing 1 mM DTT using AMARA_x000D_ peptide as a substrate (0.1 mg/ml), 20 mM_x000D_ ATP and 100 µM AMP . Reaction was_x000D_ done at 30°C for 45 min. Amount of ATP_x000D_ transferred was calculated using Kinase-_x000D_Glo reagent (Promega) |
| Purity | ≥95% |
| Shipping Information | -80°C (dry ice) |
| Size | 10 µg |
| Source / Host | insect |
| Species Reactivity | human |
| Storage | At least 6 months at -80°C. |
| Substrate / Buffer | 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol. |
| Technical Specifications | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| UniProt Number | A1: Q13131; B1: Q9Y478; G1: P54619 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
