1,10-Phenanthroline, Monohydrate

Article No BPS-27713
Product Type Proteins & Peptides
Size 0.5 ml

This product is not available for sale in your country. Please feel free to contact the help desk if you have any questions.


CAS No 5144-89-8
MW 198.2 Da
Additional Information Protect from moisture. Avoid freeze/thaw cycles.
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Description 1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn<sup>2+</sup> ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.
Supplier BPS Bioscience Inc
Notes Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.
Alias Names o-phenanthroline
Purity ≥99%
References 1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.<br>2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991).
Technical Specifications Useful for inhibition of JAMM-family isopeptidases. Especially valuable for protecting K63-linked polyubiquitin chains in cell lysates.
Product Page Updated 2019-01-04T10:01:58.434Z

Shipping info

The delivery time for this item is approximately 5-8 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.