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|Additional Information||Protect from moisture. Avoid freeze/thaw cycles.|
|Country Availability||SE, FI, DK, NO, IS, EE, LV, LT|
|Description||1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn<sup>2+</sup> ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.|
|Supplier||BPS Bioscience Inc|
|Notes||Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.|
|References||1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.<br>2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991).|
|Technical Specifications||Useful for inhibition of JAMM-family isopeptidases. Especially valuable for protecting K63-linked polyubiquitin chains in cell lysates.|
|Product Page Updated||2019-01-04T10:01:58.434Z|
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