Article No
BPS-25251
Anti-H3K4ac Polyclonal Antibody
Anti-H3K4ac Polyclonal Antibody
Antibodies Primary
Specifications
| Additional Information | Polyclonal antibody raised in rabbit against histone H3 acetylated at lysine 4 (H3K4ac), using a KLH-conjugated synthetic peptide. |
| Application | ELISA, IF, WB |
| Article No | BPS-25251 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | polyclonal |
| Clone Type | polyclonal |
| Concentration | 50 µg/34 µl |
| Conjugation | Unconjugated |
| Description | Anti-H3K4ac Polyclonal Antibody |
| Recommended Dilution | ELISA (1:500), IF (1:300), WB (1:500) |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 8350,8351,8352,8353,8354,8355,8356,8357,8358,8968 |
| Additional Information | The optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP |
| Format | Aqueous buffer solution |
| Gene Symbol | HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J |
| Immunogen | synthetic peptide |
| Notes | Polyclonal antibody raised in rabbit against histone H3 acetylated at lysine 4 (H3K4ac), using a KLH-conjugated synthetic peptide. |
| Product Type | Antibodies Primary |
| Protocol | ChIP results obtained with the antibody directed against H3K4ac ChIP assays were performed using WT and H3K4R mutant S. pombe cells, the antibody against H3K4ac (Cat. # 25251) and optimized primer pairs for qPCR. Sheared chromatin corresponding to 10 µg of DNA and 0.5 µg of antibody were used per ChIP experiment. QPCR was performed using primers specific for two different pericentric repeat regions and for the euchromatic adh1 gene. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA)._x000D_ Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K4ac (Cat. # 25251) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be 1:27,800._x000D_ Cross reactivity test using the antibody directed against H3K4ac A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K4ac (Cat. # 25251) with peptides containing other histone H3 modifications and the unmodified H3K4 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 3 shows a high specificity of the antibody for the modification of interest._x000D_ Western blot analysis using the antibody directed against H3K4ac Histone extracts (15 µg) from HeLa cells were analyzed by Western blot using the antibody directed against H3K4ac (Cat. # 25251), diluted 1:500 in TBS-Tween containing 5% BSA. The marker (in kDa) is shown on the left, the position of the protein of interest is indicated on the right._x000D_ Immunofluorescence with the antibody directed against H3K4ac Wild type and H3K4R mutant S. pombe cells were stained with both the antibody against H3K4ac (Cat. # 25251) (in red) and by Hoechst staining (in blue, left), or with the H3K4ac antibody alone (right). The antibody was used at a dilution of 1:300. |
| Purification | Affinity purified |
| Research area | Epigenetics |
| Shipping Information | -20°C or -80°C |
| Size | 50 µg |
| Source / Host | rabbit |
| Storage | Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month. |
| Substrate / Buffer | PBS containing 0.05% azide and 0.05% ProClin 300 |
| Technical Specifications | ChIP (0.5 µg/ChIP)<br> ELISA (1:500)<br> DB (1:10,000)<br> WB (1:500)<br> IF (1:300) |
| UniProt Number | P68431 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
