Article No
BPS-25216
Anti-DNMT1 Polyclonal Antibody
Anti-DNMT1 Polyclonal Antibody
Antibodies Primary
Specifications
| Additional Information | DNMT1 (UniProt/Swiss-Prot entry P26358) preferentially methylates CpG residues in hemimethylated DNA and is responsible for maintaining methylation patterns established in development. Inactivation of DNMT1 causes global demethylation and embryonic lethality. DNA methylation is coordinated with methylation of histones. Transcriptional repression by DNMT1 is mediated by direct interaction with HDAC2 |
| Application | ELISA, WB |
| Article No | BPS-25216 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | polyclonal |
| Clone Type | polyclonal |
| Concentration | 100 µl |
| Conjugation | Unconjugated |
| Description | Anti-DNMT1 Polyclonal Antibody |
| Recommended Dilution | ELISA (1:100 - 1:1000), WB (1:500) |
| Supplier | BPS Bioscience |
| Entrez Gene ID | 1786 |
| Additional Information | The optimal antibody concentration should be determined by the end-user. |
| Format | Aqueous solution |
| Gene Symbol | DNMT1 |
| Immunogen | synthetic peptide |
| Notes | Polyclonal antibody raised in rabbit against mouse DNMT1 (DNA (cytosine-5)-methyltransferase 1), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the C-terminal part of the protein |
| Alias Names | DNMT, MCMT, CXXC9, AIM |
| Product Type | Antibodies Primary |
| Protocol | Determination of the titer: To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse DNMT1 (Cat. No. 25216). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,600._x000D_ Western blot analysis using the antibody directed against DNMT1 Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse DNMT1 (Cat. No. 25216), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 183 kDa) is indicated on the right. |
| Purification | Whole serum |
| Research area | Epigenetics |
| Shipping Information | -20°C or -80°C |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | mouse |
| Storage | Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month. |
| Substrate / Buffer | Whole antiserum from rabbit containing 0.05% azide |
| Technical Specifications | ELISA (1:100 - 1:1000)<br> WB (1:500) |
| UniProt Number | P26358 |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
