Article No
BPS-25215
Anti-Cre Polyclonal Antibody
Anti-Cre Polyclonal Antibody
Antibodies Primary
Specifications
| Additional Information | Cre recombinase originates from bacteriophage P1 and recognizes loxP sites in the genome. It catalyzes recombination between two loxP sites, thereby excising the intervening DNA sequence. Cre recombinase is used as a tool for the generation of transgenic animals |
| Application | ELISA, WB |
| Article No | BPS-25215 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | polyclonal |
| Clone Type | polyclonal |
| Concentration | 100 µl |
| Conjugation | Unconjugated |
| Description | Anti-Cre Polyclonal Antibody |
| Recommended Dilution | ELISA (1:100), WB (1:500) |
| Supplier | BPS Bioscience |
| Additional Information | The optimal antibody concentration should be determined by the end-user. |
| Format | Aqueous solution |
| Immunogen | synthetic peptide |
| Notes | Polyclonal antibody raised in rabbit against Cre recombinase using a KLH-conjugated synthetic peptide located at the C-terminal part of the protein |
| Alias Names | Cre recombinase |
| Product Type | Antibodies Primary |
| Protocol | Determination of the antibody titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against Cre (cat. No. 25215) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,900._x000D_Western blot analysis using the antibody directed against Cre (1) Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the antibody against Cre (cat. No. 25215), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest (expected size: 38 kDa) is indicated on the right. |
| Purification | Whole serum |
| Research area | Epigenetics |
| Shipping Information | -20°C or -80°C |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | human |
| Storage | Store at -80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at -20°C for at least one month. |
| Substrate / Buffer | Whole antiserum from rabbit containing 0.05% azide |
| Technical Specifications | ELISA (1:100)<br> WB (1:500) |
| Product Page Updated | 2024-01-09T13:23:45.900Z |
