Article No
BIO-52056
ISOLATE II Plasmid Mini Kit
ISOLATE II Plasmid Mini Kit
Kits & Panels
Specifications
| Application | DNA Purification |
| Article No | BIO-52056 |
| Country Availability | SE, DK, NO, IS, EE, LV, LT |
| Description | ISOLATE II Plasmid Mini Kit |
| Supplier | Bioline Reagents |
| Keywords | Isolate RNA / DNA Purification Kits |
| Notes | ISOLATE II Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using silica-membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria, followed by clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants are efficiently removed during the washing steps. In the final step, highly-pure plasmid DNA is eluted. The kit shows excellent recovery of plasmid DNA and reproducible results (Fig. 1) even from low culture volumes (Fig. 2). Separate protocols are provided for the isolation of high copy and low copy plasmids. In each case, intact, high purity plasmid DNA is isolated within 25 minutes. Typically, up to 25µg and 40µg plasmid DNA is obtained from 5ml and 10ml cultures, respectively. The isolated DNA shows excellent performance in downstream molecular biology applications such as PCR, transformation, cloning, sequencing (Fig. 3), restriction analysis, etc. The online product manual has protocols for isolation of low-copy plasmids, P1 constructs and cosmids DNA from E. coli, isolation of plasmid DNA from Gram-positive bacteria as well as plasmid DNA clean-up (isolation of low-copy plasmids, P1 constructs and cosmids may require increased buffer volumes, see ordering information above for separate Plasmid Buffer Set (BIO-52078)). |
| Product Type | Kits & Panels |
| Research area | Molecular Biology |
| Size | 50 Preps |
| Product Page Updated | 2023-12-29T09:10:16.397Z |