Article No
Ab00905-3.3
Product insert
Suggested protocols
Specifications
| Application | IF, IP, WB, IHC |
| Article No | Ab00905-3.3 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | 4E5 |
| Clone Type | monoclonal |
| Description | Anti-Brg1 [4E5 ] |
| Supplier | Absolute Antibody |
| Format | Purified |
| Isotype | IgG2b |
| Notes | Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting. |
| Alias Names | alpha-BRG1; SMARCA4; BAF190; BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4 |
| Product Type | Antibodies Primary |
| Purification | Purified |
| Size | 200 µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Substrate / Buffer | PBS with 0.02% Proclin 300. |
| Technical Specifications | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| UniProt Number | P51532 |
| Product Page Updated | 2020-01-22T08:56:55.340Z |
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