Antibodies Primary


Anti-Brg1 [4E5 ]

Article No




Species Reactivity



200 µg



Source / Host



Application IF, IP, WB, IHC
Article No Ab00905-3.3
Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Clone 4E5
Clone Type monoclonal
Description Anti-Brg1 [4E5 ]
Supplier Absolute Antibody
Format Purified
Isotype IgG2b
Notes Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting.
Alias Names alpha-BRG1; SMARCA4; BAF190; BAF190A; BRG1; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; CSS4; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4
Product Type Antibodies Primary
Purification Purified
Size 200 µg
Source / Host mouse
Species Reactivity human, mouse
Storage Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Substrate / Buffer PBS with 0.02% Proclin 300.
Technical Specifications This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
UniProt Number P51532
Product Page Updated 2020-01-22T08:56:55.340Z

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