Article No
Ab00750-23.0
Specifications
| Accession Number | P10451 |
| Additional Information | This is a recombinant monoclonal antibody. |
| Application | ELISA, IHC, Inhibition |
| Article No | Ab00750-23.0 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | hu1A12 |
| Clone Type | recombinant |
| Conjugation | Unconjugated |
| Description | Anti-osteopontin [hu1A12] |
| Supplier | Absolute Antibody |
| Format | Purified |
| Isotype | IgG |
| Notes | 1A12 antibody was prepared by immunizing BALB/c mice with purified recombinant human OPN. The antibody was humanized by complementarity-determining region grafting the mouse 1A12 CDRs into a human FR template, with some FR residues being substituted for those found to be key in the mouse version to maintain affinity for human OPN. The light and heavy chain expression vectors were co-transfected into COS-7 cells. |
| Alias Names | OPN; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; SPP1; Urinary stone protein; uropontin |
| Product Type | Antibodies Primary |
| Purification | Purified |
| Research area | Cell Proliferation |
| Shipping Information | Wet Ice |
| Size | 200 µg |
| Source / Host | rabbit |
| Species Reactivity | human |
| Storage | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Substrate / Buffer | PBS with 0.02% Proclin 300. |
| Technical Specifications | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| UniProt Number | P10451 |
| Product Page Updated | 2024-03-25T11:22:07.969Z |
