Article No
ASJ-JAUY19-100
Application | ELISA, IF, IHC |
Article No | ASJ-JAUY19-100 |
Biosite Brand | Biosite |
Country Availability | all |
Clone | 10F1G12 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Anti-ALCAM antibody (405-524 aa) [10F1G12] |
Recommended Dilution | ELISA 1:10000, IF 1:50-200, IHC 1:200-1:1000 |
Supplier | Nordic Biosite |
Entrez Gene ID | 214 |
Gene Symbol | ALCAM |
Immunogen | Purified recombinant fragment of CD166 (aa405-524) expressed in E. Coli. |
Isotype | IgG1 |
Localization | Cell membrane Cell projection, axon Cell projection, dendrite. Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells . Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse . Isoform 3: Secreted |
Notes | Mouse monoclonal against CD166 |
Alias Names | Anti-Cd166 Antigen antibody,Anti-Activated Leukocyte Cell Adhesion Molecule antibody,Anti-Cd Antigen Cd166 antibody,Anti-ALCAM MEMD antibody |
Previous Article No | ASJ-7EE384 |
Product Type | Antibodies Primary |
Protocol | IHC 1:200-1:1000 ELISA 1:10000 |
Purification | Affinity Purified |
Size | 100ug |
Source / Host | mouse |
Species Reactivity | human |
Storage | -20°C |
Substrate / Buffer | Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol. |
Technical Specifications | Mouse monoclonal against CD166 |
Tissue Specificity | Detected on hematopoietic stem cells derived from umbilical cord blood. Detected on lymph vessel endothelial cells, skin and tonsil. Detected on peripheral blood monocytes. Detected on monocyte-derived dendritic cells (at protein level). Detected at low levels in spleen, placenta, liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Isoform 1 and isoform 3 are detected in vein and artery endothelial cells, astrocytes, keratinocytes and artery smooth muscle cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Detected in highly metastasizing melanoma cell lines. |
UniProt Number | Q13740 |
Product Page Updated | 2024-03-05T14:24:37.610Z |