Article No
ASB-BQFWI5-1
Mouse Anti-Phosphotyrosine-HRP
Mouse Anti-Phosphotyrosine-HRP
Antibodies Primary
Specifications
| Application | ELISA, WB, IHC, ICC |
| Article No | ASB-BQFWI5-1 |
| Biosite Brand | Biosite |
| Country Availability | all |
| Clone | PY20 |
| Clone Type | monoclonal |
| Concentration | Lot specific |
| Conjugation | HRP |
| Description | Mouse Anti-Phosphotyrosine-HRP |
| Supplier | Nordic Biosite |
| Format | Liquid |
| Immunogen | Phosphotyrosine conjugated to carrier proteins |
| Isotype | IgG2b |
| Notes | Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g., via growth factors) during normal growth and development and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation is cell surface glycoproteins. Antibodies to phosphotyrosine enable the detection, isolation, and characterization of proteins containing phosphotyrosine. The monoclonal antibody PY20 prevents internalization of activated receptors (e.g., EGFR) when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate but not by phosphoserine, phosphothreonine, or free phosphate. |
| Alias Names | pTyr20 |
| Previous Article No | ASB-IKECMT-1, ASB-140005 |
| Product Type | Antibodies Primary |
| Shipping Information | Ambient Temperature |
| Size | 1 mL |
| Source / Host | mouse |
| Storage | 2-8°C |
| Substrate / Buffer | 50% Glycerol/50% Phosphate buffered saline, pH 7.4 |
| Product Page Updated | 2024-01-11T10:51:07.446Z |
| Volume | 1.0 mL |
