Article No
AP7968c
MW | 59474 |
NCBI Number | NP_037365.2 |
Accession Number | Q9UEW8 |
Application | WB |
Article No | AP7968c |
Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
Clone | RB03418 |
Clone Type | polyclonal |
Conjugation | Unconjugated |
Description | SPAK Antibody (Center) |
Recommended Dilution | 1:1000 |
Supplier | Abgent |
Entrez Gene ID | 27347 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Isotype | IgG |
Keywords | Crown Antibodies;Primary Antibodies;Metabolism;Signal Transduction |
Notes | SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus. |
Alias Names | STE20/SPS1-related proline-alanine-rich protein kinase, Ste-20-related kinase, DCHT, Serine/threonine-protein kinase 39, STK39, SPAK |
Product Type | Antibodies Primary |
Research Area | Metabolism |
Size | 400 µl |
Source / Host | rabbit |
Species Reactivity | human, mouse |
Substrate / Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Technical Specifications | This SPAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK. |
Product Page Updated | 2023-11-23T14:38:54.086Z |