NEDD8

Article No AMB-8751
Product Type Antibodies Primary
Application ELISA, WB, IHC, ICC, FC
Size 0.1mg

Specifications

MW 9kDa
Country Availability all
BioSite Brand BioSite mAb
Clone 5B8;
Clone Type monoclonal
Description Mouse Monoclonal Antibody to NEDD8
Supplier BioSite Mab
Entrez Gene ID 4738
Immunogen Purified recombinant fragment of human NEDD8 expressed in E. Coli. ;
Isotype IgG1
Notes Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Alias Names NEDD-8
Protocol ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Source / Host mouse
Species Reactivity human
Storage 4 C, -20 C for long term storage
Substrate / Buffer Purified antibody in PBS with 0.05% sodium azide
Technical Specifications ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Product Page Updated 2018-10-15T09:15:17.802Z

Shipping info

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Source/Host:

mouse mouse

Species reactivity:

human human

Suggested protocols:

Western Blot

Western Blot

IHC-P

IHC-P