Antibodies Primary

MAP3K5

NEW
Mouse Monoclonal Antibody to MAP3K5

Article No

AMB-8661

Application

ELISA, WB, IHC, ICC, FC

Species Reactivity

human

Size

100μl

Clone

2E4;

Source / Host

mouse

Suggested protocols

Specifications

MW 55kDa
Application ELISA, WB, IHC, ICC, FC
Article No AMB-8661
BioSite Brand BioSite mAb
Clone 2E4;
Clone Type monoclonal
Description Mouse Monoclonal Antibody to MAP3K5
Supplier BioSite Mab
Entrez Gene ID 4217
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. Coli. ;
Isotype IgG1
Notes Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Alias Names ASK1; MEKK5; MAPKKK5
Product Type Antibodies Primary
Protocol ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Size 100μl
Source / Host mouse
Species Reactivity human
Storage 4°C -20°C for long term storage
Substrate / Buffer Ascitic fluid containing 0.03% sodium azide.
Technical Specifications ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400
Product Page Updated 2019-08-02T12:49:54.081Z

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