Antibodies Primary


Mouse Monoclonal Antibody to CDC27

Article No




Species Reactivity






Source / Host


Suggested protocols


MW 91kDa
Application ELISA, WB, IHC
Article No AMB-8503
BioSite Brand BioSite mAb
Country Availability all
Clone 5C12;
Clone Type monoclonal
Description Mouse Monoclonal Antibody to CDC27
Supplier Nordic BioSite
Entrez Gene ID 996
Immunogen Purified recombinant fragment of human CDC27 expressed in E. Coli. ;
Isotype IgG1
Notes Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Alias Names APC3; HNUC; ANAPC3; CDC27Hs; D0S1430E; D17S978E;
Product Type Antibodies Primary
Protocol ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000
Size 100μl
Source / Host mouse
Species Reactivity human
Storage 4°C -20°C for long term storage
Substrate / Buffer Ascitic fluid containing 0.03% sodium azide. ;
Technical Specifications ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000
Product Page Updated 2019-12-04T06:33:39.850Z

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