Article No
AMB-SA3CWV-100
NF-?B p65 Primary Antibody
NF-?B p65 Primary Antibody
Antibodies Primary
Specifications
| MW | 65kDa |
| Application | WB, ELISA |
| Article No | AMB-SA3CWV-100 |
| Biosite Brand | Biosite mAb |
| Country Availability | all |
| Clone | 6H7 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | NF-?B p65 Primary Antibody |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 5970 |
| Format | Ascitic fluid containing 0.03% sodium azide. |
| Immunogen | Purified recombinant fragment of human NF-κB p65 expressed in E. Coli. |
| Isotype | IgG1 |
| Notes | Transcription factors of the nuclear factor ? B (NF-?B)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-?B1 (p105/p50) and NF-?B2 (p100/p52). The most studied NF-?B complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-?B with its inhibitor, I?B. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I?B. This complex prevents nuclear translocation and activity of NF-?B. In response to stimuli such as cytokines, LPS, and viral infections, I?B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I?B/NF-?B complex, allowing the free heterodimeric NF-?B to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the ?B site within promoters and enhancers and functions as a transcriptional activator. |
| Alias Names | NFkappaB p65; p65; NFKB3; RELA |
| Previous Article No | AMB-8417 |
| Product Type | Antibodies Primary |
| Protocol | ELISA: 1/10000; WB: 1/500 - 1/2000 |
| Size | 100µl |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | 4°C -20°C for long term storage |
| Substrate / Buffer | Ascitic fluid containing 0.03% sodium azide. |
| Technical Specifications | ELISA: 1/10000; WB: 1/500 - 1/2000 |
| Product Page Updated | 2024-01-25T14:27:22.065Z |
