Antibodies Primary

CHK2

NEW
Mouse Monoclonal Antibody to CHK2

Article No

AMB-8271

Application

ELISA, WB, IHC, ICC

Species Reactivity

human

Size

100μl

Clone

1C12B8;

Source / Host

mouse

Suggested protocols

Specifications

MW 61kDa
Application ELISA, WB, IHC, ICC
Article No AMB-8271
BioSite Brand BioSite mAb
Clone 1C12B8;
Clone Type monoclonal
Description Mouse Monoclonal Antibody to CHK2
Supplier BioSite Mab
Entrez Gene ID 11200
Immunogen Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli. ;
Isotype IgG2b
Notes CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Names CDS1; LFS2; CHEK2
Product Type Antibodies Primary
Protocol ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000
Size 100μl
Source / Host mouse
Species Reactivity human
Storage 4°C -20°C for long term storage
Substrate / Buffer Ascitic fluid containing 0.03% sodium azide.
Technical Specifications ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000
Product Page Updated 2019-08-02T12:49:54.081Z

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