Antibodies Primary

Calreticulin

NEW
Mouse Monoclonal Antibody to Calreticulin

Article No

AMB-8233

Application

ELISA, WB

Species Reactivity

human

Size

100µl

Clone

N/A;

Source / Host

mouse

Suggested protocols

Specifications

Application ELISA, WB
Article No AMB-8233
BioSite Brand BioSite mAb
Clone N/A;
Clone Type monoclonal
Description Mouse Monoclonal Antibody to Calreticulin
Supplier BioSite Mab
Entrez Gene ID 811
Immunogen Synthetic peptide corresponding to aa(E-E-E-D-V-P-G-Q-A-K-D-E-L-C)of human Calreticulin, conjugated to KLH.
Notes Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Alias Names RO; CRT; SSA; cC1qR; FLJ26680; CALR
Product Type Antibodies Primary
Protocol ELISA: 1/10000; WB: 1/500 - 1/2000
Size 100µl
Source / Host mouse
Species Reactivity human
Storage 4°C -20°C for long term storage
Substrate / Buffer Rabbit anti-serum.
Technical Specifications ELISA: 1/10000; WB: 1/500 - 1/2000
Product Page Updated 2019-08-02T12:49:54.081Z

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