Article No
AMB-KQBW0Q-100
BRAF Primary Antibody
BRAF Primary Antibody
Antibodies Primary
Specifications
| Application | WB, ELISA |
| Article No | AMB-KQBW0Q-100 |
| Biosite Brand | Biosite mAb |
| Country Availability | all |
| Clone | 1H12F1 |
| Clone Type | monoclonal |
| Conjugation | Unconjugated |
| Description | BRAF Primary Antibody |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 673 |
| Format | Ascitic fluid containing 0.03% sodium azide. |
| Immunogen | Purified recombinant fragment of BRAF expressed in E. Coli. |
| Isotype | IgG1 |
| Notes | BRAF(V-raf murine sarcoma viral oncogene homolog B1 ) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK),it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi,primary cutaneous melanomas and colorectal cancers. |
| Alias Names | BRAF |
| Previous Article No | AMB-8172 |
| Product Type | Antibodies Primary |
| Protocol | ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000 |
| Size | 100µl |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C -20°C for long term storage |
| Substrate / Buffer | Ascitic fluid containing 0.03% sodium azide. |
| Technical Specifications | ELISA: 1/10000; WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000 |
| Product Page Updated | 2024-01-25T14:27:22.065Z |
