Article No
AG-27B-0016PF-C500
anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free)
anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free)
Antibodies Primary
Specifications
| Additional Information | anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. |
| Application | Block, ELISA, ICC |
| Article No | AG-27B-0016PF-C500 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT |
| Clone | Angy-2-1 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) |
| Supplier | Adipogen Life Sciences |
| Entrez Gene ID | 285 |
| Additional Information | anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the mouse lgG2b Fc domain. |
| Format | Liquid |
| Gene Symbol | ANGPT2 |
| Immunogen | Recombinant human angiopoietin-2. |
| Isotype | IgG2b l |
| Notes | Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.|Recombinant Antibody. Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1. Species cross-reactivity: Human, Mouse. Clone: Angy-2-1. Isotype: Mouse IgG2blambda. Applications: ELISA, FUNC (Blocking). Host: CHO cells. Liquid. In PBS containing 10% glycerol. Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Alias Names | Ang-2; Ang2; Angpt2; Agpt2 |
| Product Type | Antibodies Primary |
| Protocol | Functional: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50* = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) Inhibits the binding of human angiopoietin-2 to human Tie-2. ND50* = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) *ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). |
| Purity | >95% |
| Research area | Metabolism |
| Shipping Information | BLUE ICE |
| Size | 500 ug |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Storage | -20°C, 4°C |
| Substrate / Buffer | Liquid. In PBS containing 10% glycerol. |
| Technical Specifications | Recombinant Antibody. Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1. Species cross-reactivity: Human, Mouse. Clone: Angy-2-1. Isotype: Mouse IgG2blambda. Applications: ELISA, FUNC (Blocking). Host: CHO cells. Liquid. In PBS containing 10% glycerol. Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are closely related secreted ligands which bind with similar affinity to Tie-2. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature. Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization. Ang-2 has been identified as a secreted protein ligand of Tie-2 and has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| UniProt Number | O15123 |
| Product Page Updated | 2024-02-01T08:25:01.492Z |
