Article No
AFC-M5266J-100
Application | FC |
Article No | AFC-M5266J-100 |
Biosite Brand | Biosite Flow |
Country Availability | all |
Clone | MEM-78 |
Clone Type | monoclonal |
Conjugation | APC/Cy7 |
Description | Anti-Hu CD10 APC-Cy™7 |
Supplier | Nordic Biosite |
Entrez Gene ID | 4311 |
Format | APC-Cy™7 |
Immunogen | NALM-6 human pre-B cell line |
Isotype | IgG1 |
Notes | CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems. |
Alias Names | CALLA, Neprilysin, Neutral endopeptidase, Enkephalinase, Atriopeptidase, MME |
Product Type | Antibodies Primary |
Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Purification | Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Size | 100 tests |
Source / Host | mouse |
Species Reactivity | human |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Technical Specifications | The antibody MEM-78 reacts with CD10 antigen (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein. |
UniProt Number | P08473 |
Product Page Updated | 2024-01-03T12:18:20.318Z |