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Anti-HLA-DR PE-DyLight® 594

Antibodies Primary

Make a customized order

Article No

AFC-K08ONF-100

Application

Size

100 tests

Clone

L243

Source / Host

mouse

Species Reactivity

dog/canine

monkey

human

Article No

AFC-K08ONF-100

Application

Size

100 tests

Clone

L243

Source / Host

mouse

Species Reactivity

dog/canine

monkey

human

Specifications
Suggested protocols
References

Specifications

Application	FC
Article No	AFC-K08ONF-100
Biosite Brand	Biosite Flow
Country Availability	all
Clone	L243
Clone Type	monoclonal
Conjugation	PE/DyLight 594
Description	Anti-HLA-DR PE-DyLight® 594
Recommended Dilution	The purified antibody is conjugated with tandem dye PE-DyLight™ 594 (PE-DL594) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Supplier	Nordic Biosite
Format	PE-DyLight<sup>®</sup> 594
Immunogen	Human B lymphocytes
Isotype	IgG2a k
Keywords	CD Antigens & MHC (Veterinary), MHC (Major Histocompatibility Complex) and Related Antigens (Human)
Notes	HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
Previous Article No	AFC-0FDD2E
Product Type	Antibodies Primary
Protocol	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Purification	Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight®594 (PE-DyLight®594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Research area	Immunology
Size	100 tests
Source / Host	mouse
Species Reactivity	dog/canine, monkey, human
Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Substrate / Buffer	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Technical Specifications	The mouse monoclonal antibody L243 recognizes specifically an extracellular epitope on HLA-DR molecules, both peptide-loaded and empty.
Product Page Updated	2024-01-03T12:18:20.318Z

Suggested protocols

Flow Cytometry

References

*Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May;8(5):581-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/9149909 ,*Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May;14(5):1336-48., URL: http://www.ncbi.nlm.nih.gov/pubmed/12707403 ,*Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec;82(Pt 12):2979-87. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11714974 ,*Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies functionally distinct human regulatory T cells". J Immunol. 2008 Mar 15;180(6):3625; author reply 3626., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=comment%20on%20mhc%20class%20ii%20expression%20identifies ,*De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3491-6., URL: https://pubmed.ncbi.nlm.nih.gov/18305173/,*Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug;119(8):2143-59., URL: http://www.ncbi.nlm.nih.gov/pubmed/19620786 ,*Kalka-Moll WM, Tzianabos AO, Bryant PW, Niemeyer M, Ploegh HL, Kasper DL: Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions. J Immunol. 2002 Dec 1;169(11):6149-53., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tzianabos+ao+bryant+pw ,*Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28;278(9):7099-107., URL: http://www.ncbi.nlm.nih.gov/pubmed/12499388,,,

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