Article No
AFC-JRH8CZ-100
Anti-Hu CD235a APC-Cy™7
Anti-Hu CD235a APC-Cy™7
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-JRH8CZ-100 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | JC159 |
| Clone Type | monoclonal |
| Conjugation | APC/Cy7 |
| Description | Anti-Hu CD235a APC-Cy™7 |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 2993 |
| Format | APC-Cy™7 |
| Immunogen | Membrane preparation from splenic hairy cell leukemia |
| Isotype | IgG1 |
| Notes | CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin. |
| Alias Names | Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Purification | Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 100 tests |
| Source / Host | mouse |
| Species Reactivity | rat, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B. |
| UniProt Number | P02724 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
