Article No
AFC-0CWPBH-0.1
Anti-CD3 epsilon (activation epitope) FITC
Anti-CD3 epsilon (activation epitope) FITC
Antibodies Primary
Specifications
| Application | FC |
| Article No | AFC-0CWPBH-0.1 |
| Biosite Brand | Biosite Flow |
| Country Availability | all |
| Clone | APA1/1 |
| Clone Type | monoclonal |
| Concentration | 0.1 mg/ml |
| Conjugation | FITC |
| Description | Anti-CD3 epsilon (activation epitope) FITC |
| Recommended Dilution | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 916 |
| Format | FITC |
| Immunogen | Purified human CD3 proteins isolated from thymus |
| Isotype | IgG1 |
| Keywords | CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human) |
| Notes | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Alias Names | CD3 epsilon chain, T3E, IMD18 |
| Previous Article No | AFC-DA1859, AFC-0CWPBH-0.1 |
| Product Type | Antibodies Primary |
| Protocol | Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining. |
| Purification | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Size | 0.1 mg |
| Source / Host | mouse |
| Species Reactivity | mouse, human |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
| Substrate / Buffer | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Technical Specifications | The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation. |
| UniProt Number | P07766 |
| Product Page Updated | 2024-01-03T12:18:20.318Z |
